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Streptococcus mutansÀÇ Ä¡ÅÂÇü¼º¿¡ ´ëÇÑ Leuconostoc lactis 51ÀÇ ¿µÇâ

The effect of Leuconostoc lactis 51 against the plaque formation of Streptococcus mutans

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±èű٠( Kim Tae-Geun ) - Àü³²´ëÇб³ Ä¡°ú´ëÇÐ ¼Ò¾ÆÄ¡°úÇб³½Ç
¾ç±ÔÈ£ ( Yang Kyu-Ho ) - Àü³²´ëÇб³ Ä¡°ú´ëÇÐ ¼Ò¾ÆÄ¡°úÇб³½Ç
¿ÀÁ¾¼® ( Oh Jong-Suk ) - Àü³²´ëÇб³ ÀÇ°ú´ëÇÐ ¹Ì»ý¹°Çб³½Ç

Abstract

Ä¡¾Æ¿ì½ÄÁõÀº Ä¡¾Æ±¸Á¶ÀÇ ±¹¼ÒÀû, ħÀ±Àû, ºÐÀÚÀûÀÎ ºØ±«·Î Ư¡Áö¿öÁö´Â Ä¡¾Æ °æÁ¶Á÷¿¡ ´ëÇÑ ¼¼±Õ¼ºÁúȯÀÌ´Ù. ÀÌ·± Ä¡¾Æ¿ì½ÄÁõÀÇ ÁÖ ¿øÀαÕÀÎ S. mutansÀÇ Ä¡ÅÂÇü¼º°ú Áõ½Ä¿¡ ´ëÇÑ ¾Æµ¿ÀÇ ±¸°­¿¡¼­ ºÐ¸®µÈ L. lactis 51ÀÇ ÀÛ¿ëÀ» ¿¬±¸ÇÏ¿© ´ÙÀ½°ú °°Àº °á°ú¸¦ ¾ò¾ú´Ù.

1.ºñÄ¿ ¿ÍÀÌ¾î °Ë»ç¿¡¼­ S. mutans¿Í L. lactis 51ÀÇ È¥ÇÕ¹è¾ç½Ã S. mutans ´Üµ¶¹è¾ç¿¡ ºñÇØ Ä¡ÅÂÀÇ ¹«°Ô°¡ °¨¼ÒÇÏ¿´´Ù.

2.S. mutans´Â S. mutans¿Í L. lactis 51ÀÇ È¥ÇÕ ¹è¾ç¿¡ ºñ±³ÇÏ¿© S. mutans ´Üµ¶¹è¾ç½Ã¿¡ »ý±Õ¼ö°¡ °¨¼ÒÇÏ¿´´Ù.

3.S. mutans¿Í L. lactis 51Àº M17Y broth¿¡¼­ ´Üµ¶ ¹× È¥ÇÕ¹è¾ç½Ã ¹è¾ç 12½Ã°£ ¶§±îÁö Áõ°¡ÇÏ´Ù°¡ 24½Ã°£ ¶§¿¡ °¨¼ÒÇÏ¿´À¸³ª, M17YS broth¿¡¼­´Â S. mutans¿Í L. lactis 51ÀÇ È¥ÇÕ¹è¾ç½Ã S. mutansÀÇ »ý±Õ¼ö°¡ ½Ã°£ÀÌ Áö³²¿¡ µû¶ó °¨¼ÒÇÏ¿´´Ù.

4.L. lactis 51ÀÇ ¹è¾ç »óû¾×Àº S. mutansÀÇ Ä¡ÅÂÇü¼º°ú Áõ½Ä¿¡ ´ëÇØ ¾ïÁ¦ ÀÛ¿ëÀ» ÇÏÁö ¸øÇÏ¿´´Ù.

5.M17YS broth¿¡¼­ÀÇ L. lactis 51 ¹è¾ç »óû¾× ¼ººÐÀÇ thin layer chromatography¿¡¼­ ÀÚ´ç°ú °ú´çÀÌ °è¼Ó °ËÃâµÇ¾ú´Ù.

ÀÌ»óÀÇ °á°ú¸¦ Á¾ÇÕÇÏ¸é ±¸°­¿¡¼­ ºÐ¸®µÈ L. lactis 51´Â S. mutansÀÇ ÀΰøÄ¡Å Çü¼º°ú Áõ½ÄÀ» ¾ïÁ¦½ÃÅ°´Â °ÍÀ¸·Î »ç·áµÈ´Ù.

Dental caries is a bacterial disease of the dental hard tisssus, characterized by a localized, progressive, molecular disintegration of tooth structure. The action of Leuconostoc lactis 51 about plaque formation and replication by Streptococcus mutans was studied as follow.

1.Lower amount of plaque was produced at the mixed culture of S. mutans and L. lactis 51 than S. mutans alone on the wires in the beaker.

2.Fewer cells of S. mutans were replicated at the mixed culture of S. mutans and L. lactis 51 than S. mutans alone.

3.In M17Y broth, viable cells of S. mutans and L. lactis 51 increased for 12 hours, and decreased for 24 hours. In M17YS broth, viable cells of S. mutans showed time-dependent decrease at mixed culture of S. mutans and L. lactis 51.

4.The culture supernatant of L. lactis 51 didn¡¯t inhibit the replication of S. mutans and the formation of artificial plaque.

5. Sucrose and fructose were extracted from the culture supernatant of L. lactis 51 in M17YS broth.

Thest results suggest that L. lactis 51 isolated from the oral cavity inhibits the replication of S. mutans and the formation of artificial plaque.

Å°¿öµå

Streptococcus mutans;Ä¡ÅÂÇü¼º;Leuconostoc lactis 51
Streptococcus mutans;Leuconostoc lactis 51;Plaque formation

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